Anti-Ferritin FTH1 Rabbit Monoclonal Antibody

• Catalog Number: orb548027
• Category: Antibodies
• Description: Anti-Ferritin FTH1 Rabbit Monoclonal Antibody. Tested in WB, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
• Clonality: Monoclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
• Purification: Affinity-chromatography
• Immunogen: A synthesized peptide derived from human Ferritin
• UniProt ID: P02792, P02794
• MW: 69 kDa
• Tested applications: ICC, IF, WB
• Application notes: WB 1:500-1:2000ICC/IF 1:50-1:200. Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
• Antibody Type: Primary Antibody
• Clone Number: FBA-6
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Ferritin heavy chain; Ferritin H subunit; 1.16.3.1
• Note: For research use only

Western blot analysis of Ferritin expression in Jurkat cell lysate (orb548027). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FTH1 monoclonal antibody (Catalog # orb548027) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90503) with Tanon 5200 system. A specific band was detected for FTH1.

IF analysis of immunocytochemical section of Jurkat cells using anti-Ferritin antibody (orb548027) Ferritin was detected in immunocytochemical section. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (orb90553) for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti-Ferritin Antibody (orb548027) overnight at 4 °C. DyLight®488 Conjugated Goat AntiRabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 °C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.