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Ferritin Heavy Chain Recombinant Rabbit Monoclonal Antibody

Catalog Number: orb1607972

DispatchUsually dispatched within 5-10 working days
$ 270.00
Catalog Numberorb1607972
CategoryAntibodies
DescriptionFerritin Heavy Chain Recombinant Rabbit Monoclonal Antibody
Species/HostRabbit
ClonalityRecombinant
Clone Number5B5
Tested applicationsIF, IHC-Fr, IHC-P, WB
Predicted ReactivityHuman, Mouse, Rat
ReactivityHuman
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human Ferritin Heavy Chain
Antibody TypeRecombinant Antibody
Concentration1mg/ml
Dilution rangeWB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Form/AppearanceLiquid
ConjugationUnconjugated
MW20 kDa
TargetFTH1
UniProt IDP02794
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesApoferritin; Cell proliferation inducing gene 15 p
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NoteFor research use only
Expiration Date12 months from date of receipt.
Ferritin Heavy Chain Recombinant Rabbit Monoclonal Antibody

ICC staining of Ferritin Heavy Chain in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (orb1607972, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1000 dilution. The nuclear counter stain is DAPI (blue).

Ferritin Heavy Chain Recombinant Rabbit Monoclonal Antibody

Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-Ferritin Heavy Chain antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (orb1607972, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Ferritin Heavy Chain Recombinant Rabbit Monoclonal Antibody

Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-Ferritin Heavy Chain antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (orb1607972, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.06.

Ferritin Heavy Chain Recombinant Rabbit Monoclonal Antibody

Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Ferritin Heavy Chain antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (orb1607972, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Ferritin Heavy Chain Recombinant Rabbit Monoclonal Antibody

Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Ferritin Heavy Chain antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (orb1607972, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Ferritin Heavy Chain Recombinant Rabbit Monoclonal Antibody

Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Ferritin Heavy Chain antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (orb1607972, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Ferritin Heavy Chain Recombinant Rabbit Monoclonal Antibody

Western blot analysis of Ferritin Heavy Chain on human liver tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (orb1607972, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5000 dilution was used for 1 hour at room temperature.