Anti-ERK5 Antibody

• Catalog Number: orb323265
• Category: Antibodies
• Description: Rabbit polyclonal antibody to ERK5
• Target: MAPK7
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Immunogen: Recombinant protein corresponding to human ERK5.
• UniProt ID: Q13164
• Tested applications: IH, WB
• Dilution range: WB: 1:1000-1:2000, IHC-P: 1:200-1:500
• Antibody Type: Primary Antibody
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti BMK1 antibody, anti ERK5 antibody, anti PRKM7
• Note: For research use only
• Entrez: 5598

Western blot analysis of ERK5 expression in Hela (A), 293T (B), NIH3T3 (C), mouse skeletal muscle (D), rat kidney (E), rat skeletal muscle (F) whole cell lysates. (Predicted band size: 88 kD; Observed band size: 89-115 kD)

Immunohistochemical analysis of ERK5 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.