Phospho-ERK5 (Ser496) Rabbit Polyclonal Antibody

• Catalog Number: orb5183
• Category: Antibodies
• Description: Phospho-ERK5 (Ser496) Rabbit Polyclonal Antibody
• Target: MAPK7
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: affinity purified by Protein A
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human ERK5 around the phosphorylation site of Ser496 GP(p-S)AP
• UniProt ID: Q13164
• RRID: AB_10934187
• MW: 90 kDa
• Tested applications: ICC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: ERK5(phospho S496); Big MAP kinase 1; BMK 1; BMK 1
• Note: For research use only

References

Arianna Filippelli 1, Valerio Ciccone 1, Cinzia Del Gaudio 1, Vittoria Simonis 1, Maria Frosini 1, Ignazia Tusa 2, Alessio Menconi 2, Elisabetta Rovida 3, Sandra Donnini ERK5 mediates pro-tumorigenic phenotype in non-small lung cancer cells induced by PGE2 Biochim Biophys Acta Mol Cell Res, (2024)

PubMed: 39128596

Applications

WB

Reactivity

Human

Sample: Lane 1: HepG2 (Human) Cell Lysate at 30 ug, Lane 2: Hcclm3 (Human) Cell Lysate at 30 ug, Primary: Anti-phospho-ERK5 (Ser496) (orb5183) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 115 kD, Observed band size: 115 kD.

Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (phospho-ERK5 (Ser496)) polyclonal Antibody, Unconjugated (orb5183) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-ERK5 (Ser496)) Polyclonal Antibody, Unconjugated (orb5183) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Tissue/Cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-phospho-ERK5 (Ser496) Polyclonal Antibody, Unconjugated (orb5183) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: human myometrium tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-phospho-ERK5 (Ser496) Polyclonal Antibody, Unconjugated (orb5183) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.