Anti-ERK3 Antibody

• Catalog Number: orb323264
• Category: Antibodies
• Description: Rabbit polyclonal antibody to ERK3
• Target: MAPK6
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Immunogen: Recombinant protein corresponding to human ERK3.
• UniProt ID: Q16659
• Tested applications: IH, WB
• Dilution range: WB: 1:500-1:1000, IHC-P: 1:100-1:200
• Antibody Type: Primary Antibody
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti ERK3 antibody, anti PRKM6 antibody, anti Mito
• Note: For research use only
• Entrez: 5597
• Expiration Date: 12 months from date of receipt.

Western blot analysis of ERK3 expression in Hela (A), 293T (B), NIH3T3 (C), rat brain (D) whole cell lysates. (Predicted band size: 82 kD; Observed band size: 83-105 kD)

Immunohistochemical analysis of ERK3 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.