Anti-ERK1/2 (Phospho-T202/Y204) Antibody

• Catalog Number: orb315802
• Category: Antibodies
• Description: Rabbit polyclonal antibody to ERK1/2
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T202/Y204 of human ERK1/2 protein. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Dilution range: WB: 1-500:1000, IHC-P: 1-100:200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: MAPK1; MAPK3
• Entrez: 26417, 50689, 5594, 116590, 5595, 26413
• UniProt ID: Q63844, P28482, P21708, P27361, P63085, P63086
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: Anti-ERK 1 antibody, anti-ERK 2 antibody, anti-ERK
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of ERK1/2 (Phospho-T202/Y204) expression in A549 (A), Panc1 (B), rat lung (C), AML12 (D) whole cell lysates. (Predicted band size: 43; 41 kD; Observed band size: 44; 42 kD)

Immunohistochemical analysis of ERK1/2 (Phospho-T202/Y204) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.