ERCC1 Antibody

SKU: orb323139

Description

The ERCC1 Antibody is suitable for IHC, WB. It is a Monoclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence of human ERCC1. The exact sequence is proprietary. Purification: Affinity chromatography

Images & Validation

• Tested Applications: IHC, WB
• Dilution range: WB: 1:1000-1:2000, IHC-P: 1:100-1:200
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence of human ERCC1. The exact sequence is proprietary.
• Purification: Affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Disclaimer: For research use only

Alternative Names

DNA excision repair protein ERCC-1

Western blot analysis of ERCC1 expression in Hela (A), HepG2 (B), 293T (C), Jurkat (D) whole cell lysates. (Predicted band size: 32 kD; Observed band size: 36 kD)

Immunohistochemical analysis of ERCC1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Quick Database Links

UniProt

P07992

NCBI Gene ID

2067