ERCC1 Antibody (C-term)

• Catalog Number: orb1927426
• Category: Antibodies
• Description: Mouse Monoclonal Antibody (Mab)
• Target: This ERCC1 antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 268-297 amino acids from the C-terminal region of human ERCC1.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgM,k
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Euglobin precipitation followed by dialysis against PBS.
• UniProt ID: P07992
• MW: 32562 Da
• Tested applications: WB
• Dilution range: WB: 1:2000, WB: 1：1000, WB: 1:1000
• Clone Number: 752CT13.2.5
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: DNA excision repair protein ERCC-1, ERCC1
• Note: For research use only
• NCBI: NP_001974.1, NP_973730.1, NP_001159521.1

ERCC1 Antibody (C-term) western blot analysis in Hela, A549 cell line lysates (35μg/lane).This demonstrates the ERCC1 antibody detected the ERCC1 protein (arrow).

Western blot analysis of lysates from A549, T47D cell line (from left to right), using ERCC1 Antibody (C-term). Diluted at 1:1000 at each lane. A goat anti-mouse IgM H&L (HRP) at 1: 3000 dilution was used as the secondary antibody. Lysates at 20 μg per lane.

All lanes: Anti-ERCC1 Antibody (C-term) at 1:2000 dilution. Lane 1: A549 whole cell lysates. Lane 2: Hela whole cell lysates. Lane 3: T47D whole cell lysates. Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgM, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 33 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.