EIF5 Antibody

• Catalog Number: orb412468
• Category: Antibodies
• Description: Rabbit polyclonal antibody to EIF5
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: Recombinant fusion protein of human EIF5
• UniProt ID: P55010, Q07205, P59325
• Tested applications: IF, WB
• Dilution range: WB: 1:500-2000, IHC-P: 1:50-200, IF/ICC: 1:50-200
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Eukaryotic translation initiation factor 5; eIF-5
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 217869, 1983, 102641436, 56783
• Expiration Date: 12 months from date of receipt.

Western blot analysis of EIF5 expression in MCF7 (A), Hela (B) whole cell lysates. (Predicted band size: 49 kD; Observed band size: 55 kD)

Immunofluorescent analysis of EIF5 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.