EGFR Antibody (PE)

• Catalog Number: orb154516
• Category: Antibodies
• Description: Mouse monoclonal antibody conjugated to PE which recognizes EGFR that belongs to the protein kinase superfamily, Tyr protein kinase family. EGFR is involved in organ morphogenesis and maintenance and repair of tissues, but upregulation of EGFR is associated with tumor progression. The EGF receptor family comprises several related receptor tyrosine kinases that are frequently overexpressed in a variety of carcinomas.
• Target: EGFR
• Clonality: Monoclonal
• Isotype: Mouse IgG2b kappa
• Conjugation: PE
• Reactivity: Equine, Human
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Immunogen: Human epidermoid carcinoma line A431
• UniProt ID: P00533
• Tested applications: FC
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
• Antibody Type: Primary Antibody
• Clone Number: EGFR1
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Alternative names: EGF-R, Her1, ERBB1, PIG61, NISBD2
• Research Area: Cancer, Developmental Biology, Epigenetics, Infect
• Note: For research use only
• Entrez: 1956
• Expiration Date: 12 months from date of receipt.

Flow cytometry analysis (surface staining) of EGFR on A431 cells with anti-EGFR (EGFR1) PE.