EGFR Rabbit Polyclonal Antibody (PE)

• Catalog Number: orb124601
• Category: Antibodies
• Description: EGFR Rabbit Polyclonal Antibody (PE)
• Target: EGFR
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: PE
• Reactivity: Human, Rat
• Predicted Reactivity: Canine, Mouse, Porcine
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human EGFR (951-1050/1210aa)
• Tested applications: FC, IF
• Dilution range: Flow-Cyt=1μg/Test, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Alternative names: EGFR; Avian erythroblastic leukemia viral(v erb b)
• Research Area: Cancer Research, Cell Biology, Cell Differentiatio
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: A431. Primary Antibody (green line): Rabbit Anti-EGFR antibody (orb124601), Dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Blank control: A549. Primary Antibody (green line): Rabbit Anti-EGFR antibody (orb124601), Dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Blank control: Hela. Primary Antibody (green line): Rabbit Anti-Cytokeratin 18 antibody, Dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.