Anti-EGFR domain III [h-R3 (Nimotuzumab)]

SKU: orb613908

Description

Human monoclonal antibody to EGFR domain III

Research Area

Cancer Biology

Images & Validation

• Tested Applications: Blocking, FC, IHC
• Reactivity: Human

Key Properties

• Host: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: h-R3 (Nimotuzumab)
• Immunogen: This antibody we generated by humanisation of the murine antibody R3, raised against EGFR purified from placenta membranes and A431 tumors.
• Target: EGFR domain III
• Purity: Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ERBB-D3; ERBB1; HER1; PIG61; mENA; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; proto-oncogene c-ErbB-1; receptor tyrosine-protein kinase erbB-1; epidermal growth factor

Immunofluorescence staining of fixed HeLa cells with anti-EGFR domain III antibody h-R3 (Nimotuzumab). Immunofluorescence analysis of unpermeabilisd paraformaldehyde fixed HeLa cells on Shi-fix™ coverslips stained with the chimeric rabbit version of h-R3 (orb1152505) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb1152505, DAPI, merged channels and an isotype control. The isotype control was stained with unimmunised rabbit IgG followed by Alexa Fluor® 488 secondary antibody.

Quick Database Links

Gene Symbol

EGFR domain III

UniProt

P00533