Anti-EGFR domain III [h-R3 (Nimotuzumab)]

• Catalog Number: orb1152506
• Category: Antibodies
• Description: Mouse monoclonal antibody to EGFR domain III
• Target: EGFR domain III
• Clonality: Monoclonal
• Species/Host: Human
• Isotype: Human IgG2a
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Purity: Purified
• Immunogen: This antibody we generated by humanisation of the murine antibody R3, raised against EGFR purified from placenta membranes and A431 tumors.
• UniProt ID: P00533
• Tested applications: Blocking, FC, IHC
• Clone Number: h-R3 (Nimotuzumab)
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: ERBB-D3; ERBB1; HER1; PIG61; mENA; avian erythrobl
• Research Area: Cancer Biology
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of fixed HeLa cells with anti-EGFR domain III antibody h-R3 (Nimotuzumab). Immunofluorescence analysis of unpermeabilisd paraformaldehyde fixed HeLa cells on Shi-fix™ coverslips stained with the chimeric rabbit version of h-R3 (orb1152505) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb1152505, DAPI, merged channels and an isotype control. The isotype control was stained with unimmunised rabbit IgG followed by Alexa Fluor® 488 secondary antibody.