EF-2 (phospho Thr56) rabbit pAb

• Catalog Number: orb767919
• Category: Antibodies
• Description: Rabbit polyclonal antibody to EF-2 (phospho-Thr56)
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Immunogen: The antiserum was produced against synthesized peptide derived from human eEF2 around the phosphorylation site of Thr56. AA range:31-80
• MW: 100
• Tested applications: ELISA, IF, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-EEF2 antibody, anti-EF2 antibody, anti-Elonga
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot analysis of SKOV3 cells using Phospho-EF-2 (T56) Polyclonal Antibody diluted at 1:2000.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using eEF2 (Phospho-Thr56) Antibody.

Immunofluorescence analysis of HUVEC cells, using eEF2 (Phospho-Thr56) Antibody. The picture on the right is blocked with the phospho peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using eEF2 (Phospho-Thr56) Antibody. The picture on the right is blocked with the phospho peptide.