E Cadherin Antibody

SKU: orb323260

Description

The E Cadherin Antibody is suitable for IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against Recombinant protein corresponding to human E Cadherin. Purification: The antibody was purified by immunogen affinity chromatography.

Research Area

Epigenetics

Images & Validation

• Tested Applications: IHC, WB
• Dilution range: WB: 1:1000-1:2000, IHC-P: 1:200-1:500
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant protein corresponding to human E Cadherin.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Disclaimer: For research use only

Alternative Names

CDHE; UVO; Cadherin-1; CAM 120/80; Epithelial cadherin; E-cadherin; Uvomorulin; CD324

Western blot analysis of E Cadherin expression in Hela (A) whole cell lysates. (Predicted band size: 97 kD; Observed band size: 135 kD)

Immunohistochemical analysis of E Cadherin staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Quick Database Links

UniProt

P12830

NCBI Gene ID

999

References

Chalcarz, Micha? et al. The absence of early malignant changes in women subjected to Aquafilling breast augmentation on the basis of E-cadherin and N-cadherin immunohistochemical expression Cent Eur J Immunol, 47, 350-356 (2022)

PubMed: 36817402