E Cadherin Mouse Monoclonal Antibody

SKU: orb1474005

Description

The E Cadherin Antibody is suitable for IHC, WB. It is a Monoclonal, Unconjugated antibody which raised against Recombinant fusion protein of human E Cadherin. The exact sequence is proprietary. Purification: This antibody is purified through a protein G column.

Images & Validation

• Tested Applications: IHC, WB
• Dilution Range: WB (1/1000 - 1/2000), IH (1/50 - 1/200)
• Reactivity: Human, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Immunogen: Recombinant fusion protein of human E Cadherin. The exact sequence is proprietary.
• Target: CDH1
• Purification: This antibody is purified through a protein G column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CDHE; UVO; Cadherin-1; CAM 120/80; Epithelial cadherin; E-cadherin; Uvomorulin; CD324

Western blot analysis of E Cadherin expression in SW480 (A), MCF7 (B), WiDr (C), mouse stomach (D) whole cell lysates. (Predicted band size: 97 kD; Observed band size: 128 kD)

Immunohistochemical analysis of E Cadherin staining in human liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Quick Database Links

Gene Symbol

CDH1

UniProt

P12830, P09803

NCBI Gene ID

999, 12550