DKK2 Antibody

SKU: orb666488

Description

The DKK2 Antibody is suitable for IF, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide of human DKK2. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Research Area

Developmental Biology, Epigenetics, Neuroscience, Signaling Pathways, Wnt

Images & Validation

• Tested Applications: IF, WB
• Dilution range: WB: 1-500-2000
• Reactivity: Human, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-conjugated synthetic peptide of human DKK2. The exact sequence is proprietary.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Disclaimer: For research use only

Alternative Names

Dickkopf-related protein 2; Dickkopf-2; Dkk-2; hDkk-2

Western blot analysis of DKK2 expression in A549 (A) whole cell lysates. (Predicted band size: 28 kD; Observed band size: 30 kD)

Immunofluorescent analysis of DKK2 staining in human skin cancer. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Quick Database Links

UniProt

Q9UBU2, Q9QYZ8

NCBI Gene ID

56811, 27123