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Catalog Number | orb1935274 |
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Category | Antibodies |
Description | Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB2539, RB2540 |
Tested applications | IHC-P, WB |
Reactivity | Human, Mouse |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:1000, WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Conjugation | Unconjugated |
MW | 28447 Da |
Target | This DKK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 31-62 amino acids from the N-terminal region of human DKK2. |
UniProt ID | Q9UBU2 |
NCBI | NP_055236.1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Dickkopf-related protein 2, Dickkopf-2, Dkk-2, hDk Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
The anti-DKK2 Pab is used in Western blot to detect DKK2 in Jurkat cell lysate.
DKK2 Antibody (T46) western blot analysis in mouse brain tissue lysates (35 ug/lane). This demonstrates the DKK2 antibody detected the DKK2 protein (arrow).
Western blot analysis of DKK2 (arrow) using DKK2 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the DKK2 gene (Lane 2).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.