Anti-DDX53 Antibody

• Catalog Number: orb1879522
• Category: Antibodies
• Description: Rabbit polyclonal antibody to DDX53
• Clonality: Polyclonal
• Tested applications: FC, WB
• Reactivity: Human
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-terminal region of human DDX53. The exact sequence is proprietary.
• Dilution range: WB (1/500 - 1/1000), FC (1/10 - 1/50)
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Entrez: 168400
• UniProt ID: Q86TM3
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Alternative names: CAGE; Probable ATP-dependent RNA helicase DDX53; C
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of DDX53 expression in DU145 (A), RL952 (B) whole cell lysates. (Predicted band size: 71 kD; Observed band size: 71 kD)

Flow cytometric analysis of WiDr cells using Anti-DDX53 Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Rabbit IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.