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DDX53 Antibody (C-term)
Catalog Number: orb1929213
| Catalog Number | orb1929213 |
|---|---|
| Category | Antibodies |
| Description | Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB18086 |
| Tested applications | FC, WB |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | WB: 1:2000, FC: 1:10~50 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Conjugation | Unconjugated |
| MW | 71154 Da |
| Target | This DDX53 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 597-626 amino acids from the C-terminal region of human DDX53. |
| UniProt ID | Q86TM3 |
| NCBI | NP_874358.2 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Probable ATP-dependent RNA helicase DDX53, Cancer- Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Flow cytometric analysis of WiDr cells using DDX53 Antibody (C-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
All lanes: Anti-DDX53 Antibody (C-term) at 1:2000 dilution. Lane 1: DU145 whole cell lysate. Lane 2: RL95-2 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 71 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
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