Anti-DARC [2C3]

SKU: orb613953

Description

Rabbit monoclonal antibody to DARC

Research Area

Immunology & Inflammation, Infectious Disease & Virology

Images & Validation

• Tested Applications: Blocking, ELISA, FC
• Reactivity: Human

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG
• Clone No.: 2C3
• Immunogen: CHO cells transfected with cDNA encoding the Duffy glycoprotein.
• Target: DARC
• Purity: Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Fy6; Duffy Antigen Receptor for Chemokines; NaM185-2C3

Immunofluorescence staining of fixed HepG2 cells with anti-DARC antibody 2C3. Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells on Shi-fix™ coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of 2C3 (orb613953) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 µg/ml), showing cytoplasmic staining, likely of endosomes, and some membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb613953, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor® 488 secondary antibody.

Western Blot using anti-DARC antibody 2C3 Human liver lysate samples (35 µg protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of 2C3 (orb613953) at 0.03 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for DARC is 35.6kDa. orb613953 successfully detected DARC in human liver lysate.

Quick Database Links

Gene Symbol

DARC

UniProt

Q4VBN9