Anti-DARC [2C3]

• Catalog Number: orb613952
• Category: Antibodies
• Description: Mouse monoclonal antibody to DARC
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 2C3
• Tested applications: Blocking, ELISA, FC
• Reactivity: Human
• Isotype: IgG1
• Immunogen: CHO cells transfected with cDNA encoding the Duffy glycoprotein.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: DARC
• UniProt ID: Q4VBN9
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Fy6; Duffy Antigen Receptor for Chemokines; NaM185
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of fixed HepG2 cells with anti-DARC antibody 2C3. Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells on Shi-fix™ coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of 2C3 (orb613953) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 µg/ml), showing cytoplasmic staining, likely of endosomes, and some membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb613953, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor® 488 secondary antibody.

Western Blot using anti-DARC antibody 2C3 Human liver lysate samples (35 µg protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of 2C3 (orb613953) at 0.03 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for DARC is 35.6kDa. orb613953 successfully detected DARC in human liver lysate.