Cytokeratin 19 Antibody (Biotin)

SKU: orb43667

Description

Mouse Monoclonal to Cytokeratin 19.

Images & Validation

• Tested Applications: FC, ICC, IHC-P, IP, WB
• Reactivity: Human
• Application Notes:
  Western blotting: Recommended dilution: 1-2 μg/ml.Flow cytometry: Recommended dilution: 4-10 μg/ml. Intracellular staining.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Clone No.: BA-17
• Immunogen: Mammary organoids.
• Target: Cytokeratin 19
• Purification: Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
• Conjugation: Biotin

Storage & Handling

• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

K19, CK19, CYK19, KRT19, K1CS

Western blotting analysis of human cytokeratin 19 using mouse monoclonal antibody BA-17 on lysates of HT-29 cell line and MOLT-4 cell line (cytokeratin non-expressing cell line; negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of biotinylated mouse anti-cytokeratin 19 monoclonal antibody followed by IRDye800-conjugated streptavidin. Cytokeratin 19 was detected at approximately 41 kDa.

Quick Database Links

Gene Symbol

Cytokeratin 19

UniProt

P08727

NCBI Gene ID

3880