Cytokeratin 19 Antibody (Biotin)

• Catalog Number: orb43634
• Category: Antibodies
• Description: Mouse Monoclonal to Cytokeratin 19.
• Target: Cytokeratin 19
• Clonality: Monoclonal
• Isotype: Mouse IgG2a
• Conjugation: Biotin
• Reactivity: Human
• Concentration: 1 mg/ml
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Purification: Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
• Immunogen: MCF-7 human breast adenocarcinoma cell line
• UniProt ID: P08727
• RRID: AB_10993496
• Tested applications: ELISA, FC, ICC, IHC-P, IP, WB
• Application notes: Flow cytometry: Recommended dilution: 1-5 μg/ml, intracellular staining.Western blotting: Recommended dilution: 1-2 μg/ml.
• Antibody Type: Primary Antibody
• Clone Number: A53-B/A2
• Storage: Store at 2-8°C. Do not freeze.
• Alternative names: K19, CK19, CYK19, KRT19, K1CS
• Research Area: Epigenetics, Infectious Diseases
• Note: For research use only
• Entrez: 3880

Western blotting analysis of human cytokeratin 19 using mouse monoclonal antibody A53-B/A2 on lysates of HT-29 cell line and MOLT-4 cell line (cytokeratin non-expressing cell line; negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of biotinylated mouse anti-cytokeratin 19 monoclonal antibody followed by IRDye800-conjugated streptavidin. Cytokeratin 19 was detected at approximately 41 kDa.