Cytokeratin 18 Antibody (Biotin)

SKU: orb43747

Description

Mouse monoclonal antibody against Cytokeratin 18 conjugated to Biotin

Images & Validation

• Tested Applications: ELISA, ICC, IHC-P, IP, WB
• Reactivity: Human
• Application Notes:
  Western blotting: Recommended dilution: 1-2 μg/ml.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: DA-7
• Immunogen: Human breast carcinoma cell line PMC-42.
• Target: Cytokeratin 18
• Purification: Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
• Conjugation: Biotin

Storage & Handling

• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

K18, CK18, CYK18, KRT18

Western blotting analysis of human cytokeratin 18 using mouse monoclonal antibody DA-7 on lysates of HT-29 cell line and MOLT-4 cell line (cytokeratin non-expressing cell line; negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of biotinylated mouse anti-cytokeratin 18 monoclonal antibody followed by IRDye800-conjugated streptavidin. Cytokeratin 18 was detected at approximately 46 kDa.

Quick Database Links

Gene Symbol

Cytokeratin 18

UniProt

P05783

NCBI Gene ID

3875