Cytokeratin 18 Antibody (Biotin)

• Catalog Number: orb43703
• Category: Antibodies
• Description: Mouse monoclonal antibody against Cytokeratin 18 conjugated to Biotin
• Target: Cytokeratin 18
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Conjugation: Biotin
• Reactivity: Mammal
• Concentration: 1 mg/ml
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Purification: Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
• Immunogen: Cytoskeleton preparation of epidermal carcinoma cell line A431.
• UniProt ID: P05783
• RRID: AB_10997290
• Tested applications: ELISA, FC, ICC, IHC-P, IP, WB
• Application notes: Flow cytometry: Recommended dilution: 1-4 μg/ml. Intracellular staining.Western blotting: Recommended dilution: 1-2 μg/ml.
• Antibody Type: Primary Antibody
• Clone Number: C-04
• Storage: Store at 2-8°C. Do not freeze.
• Alternative names: K18, CK18, CYK18, KRT18
• Research Area: Epigenetics, Infectious Diseases
• Note: For research use only
• Entrez: 3875

Western blotting analysis of human cytokeratin 18 using mouse monoclonal antibody C-04 on lysates of HT-29 cell line and MOLT-4 cell line (cytokeratin non-expressing cell line; negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of biotinylated mouse anti-cytokeratin 18 monoclonal antibody followed by IRDye800-conjugated streptavidin. Cytokeratin 18 was detected at approximately 46 kDa, and its proteolytic fragment at approximately 25 kDa.