Cytokeratin 18 Antibody (Biotin)

SKU: orb43703

Description

Mouse monoclonal antibody against Cytokeratin 18 conjugated to Biotin

Images & Validation

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Tested Applications	ELISA, FC, ICC, IHC-P, IP, WB
Reactivity	Mammal
Application Notes	Flow cytometry: Recommended dilution: 1-4 μg/ml. Intracellular staining.Western blotting: Recommended dilution: 1-2 μg/ml.

Key Properties

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Antibody Type	Primary Antibody
Clonality	Monoclonal
Isotype	Mouse IgG1
Clone No.	C-04
Immunogen	Cytoskeleton preparation of epidermal carcinoma cell line A431.
Target	Cytokeratin 18
Purification	Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
Conjugation	Biotin

Storage & Handling

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Storage	Store at 2-8°C. Do not freeze.
Buffer/Preservatives	Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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K18, CK18, CYK18, KRT18

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Western blotting analysis of human cytokeratin 18 using mouse monoclonal antibody C-04 on lysates of HT-29 cell line and MOLT-4 cell line (cytokeratin non-expressing cell line; negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of biotinylated mouse anti-cytokeratin 18 monoclonal antibody followed by IRDye800-conjugated streptavidin. Cytokeratin 18 was detected at approximately 46 kDa, and its proteolytic fragment at approximately 25 kDa.