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Catalog Number | orb43721 |
---|---|
Category | Antibodies |
Description | Mouse Monoclonal to Cyclin D1. |
Target | Cyclin D1 |
Clonality | Monoclonal |
Isotype | Mouse IgG1 |
Conjugation | Unconjugated |
Reactivity | Human, Rat |
Concentration | 1 mg/ml |
Buffer/Preservatives | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Purity | Purified by protein-A affinity chromatography. |
Purification | Purified by protein-A affinity chromatography. |
Immunogen | Purified cyclin D1 protein |
UniProt ID | P24385 |
RRID | AB_10996175 |
Tested applications | ELISA, FC, ICC, IHC-Fr, IHC-P, IP, WB |
Dilution range | Immunohistochemistry (frozen sections): Recommended dilution: 2 μg/ml; positive tissue: colon. Western blotting: Recommended dilution: 1 μg/ml. Immunocytochemistry: Recommended dilution: 1 μg/ml. Immunoprecipitation: Recommended dilution: 1 μg/ml. Immunohistochemistry (paraffin sections): Heat treatment, sodium citrate buffer pH 6.0. Flow cytometry: Recommended dilution: 4-10 µg/ml. Intracellular staining. |
Application notes | Immunohistochemistry (frozen sections): Recommended dilution: 2 μg/ml; positive tissue: colon.Western blotting: Recommended dilution: 1 μg/ml.Immunocytochemistry: Recommended dilution: 1 μg/ml. Immunoprecipitation: Recommended dilution: 1 μg/ml.Immunohistochemistry (paraffin sections): Heat treatment, sodium citrate buffer pH 6.0.Flow cytometry: Recommended dilution: 4-10 µg/ml. Intracellular staining. |
Antibody Type | Primary Antibody |
Clone Number | CD1.1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | Anti-Cyclin D1 antibody |
Note | For research use only |
Entrez | 595 |
FC, ICC, IHC-Fr, IHC-P, IP, WB | |
Human, Mouse, Primate, Rat | |
Monoclonal | |
Unconjugated |