Anti-Cyclin D1 (Phospho-T286) Antibody

• Catalog Number: orb304712
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Cyclin D1 (phospho-T286)
• Target: CCND1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T286 of human Cyclin D1 protein. The exact sequence is proprietary.
• UniProt ID: P24385, P39948, P25322
• Tested applications: IH, WB
• Dilution range: WB: 1:500-1000, IHC-P: 1:100-200
• Antibody Type: Primary Antibody
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti BCL1 antibody, anti PRAD1 antibody, anti G1/S
• Note: For research use only
• Entrez: 595, 58919, 12443

Western blot analysis of Cyclin D1 (Phospho-T286) expression in MCF7 EGF-treated (A), rat muscle (B) whole cell lysates. (Predicted band size: 33 kD; Observed band size: 36 kD)

Immunohistochemical analysis of Cyclin D1 (Phospho-T286) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.