Phospho-Cyclin D1 (Thr286) Rabbit Polyclonal Antibody

• Catalog Number: orb5929
• Category: Antibodies
• Description: Phospho-Cyclin D1 (Thr286) Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC
• Predicted Reactivity: Bovine, Canine, Mouse, Sheep
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human Cyclin D1 around the phosphorylation site of Thr286 AC(p-T)PT
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: ICC/IF=1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 34 kDa
• Target: CCND1
• UniProt ID: P24385
• RRID: AB_10920256
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Cyclin D1(phospho T286); p-Cyclin D1(phospho T286)
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Tissue/Cell: A431 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Phospho-Cyclin D1 (Thr286)) polyclonal Antibody, Unconjugated (orb5929) 1:100, 90 minutes at 37°C, followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Tissue/Cell: SH-SY5Y cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Phospho-Cyclin D1 (Thr286)) polyclonal Antibody, Unconjugated (orb5929) 1:100, 90 minutes at 37°C, followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.