CRKII Rabbit Monoclonal Antibody

• Catalog Number: orb866497
• Category: Antibodies
• Description: CRKII Rabbit Monoclonal Antibody
• Clonality: Monoclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 0.5mg/ml
• Purification: Affinity-chromatography
• Immunogen: A synthesized peptide derived from human CRKII
• UniProt ID: P46108
• MW: 38 kDa
• Tested applications: FC, ICC, IF, IP, WB
• Application notes: WB 1:500-2000ICC/IF 1:50-200IP 1:50FC 1:100. Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
• Antibody Type: Primary Antibody
• Clone Number: 24C53
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Proline-, glutamic acid- and leucine-rich protein
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of CRKII expression in K562 cell lysate.

IF analysis of CRKII using anti-CRKII antibody (orb866497) and anti-Beta Tubulin antibody (orb623835). CRKII was detected in immunocytochemical section of HELA cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (orb90553) for 15 mins. The cells were blocked with 10% goat serum. And then incubated at 1: 50 with rabbit anti-CRKII Antibody (orb866497) and mouse anti-Beta Tubulin antibody (orb623835) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (orb27705) and DyLight®488 Conjugated Goat Anti-Mouse IgG were used as secondary antibody at 1: 500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.