Phospho-CRK (Tyr221) Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb2562489
• Category: Antibodies
• Description: Phospho-CRK (Tyr221) Recombinant Rabbit Monoclonal Antibody
• Target: CRK
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Human
• Form/Appearance: Liquid
• Buffer/Preservatives: 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% rAlbumin, 0.02% Proclin300 and 50% glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: A synthesized peptide derived from human CRK around the phosphorylation site of Y221 GP-pY-AQ 216-226/304
• UniProt ID: P46108
• MW: 42 kDa
• Tested applications: ICC, WB
• Dilution range: WB=1:1000-2000, ICC/IF=1:50-200
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Adapter molecule crk; avian sarcoma virus CT10(v-c
• Research Area: Cell Biology, Cytoplasmic, MAPK/ERK Signalling Pat
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of (1) K562 cell lysate treated with AP, (2) K562 cell lysate. Using Phospho-CRK (Tyr221) (orb2562489) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.