Creatine Kinase M Rabbit Polyclonal Antibody

SKU: orb213745

Description

The Creatine Kinase M Antibody is suitable for IF, IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Creatine Kinase M. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Research Area

Pharmacology & Drug Discovery

Images & Validation

• Tested Applications: IF, IHC, WB
• Dilution Range: WB: 1-500-1-1000, IHC-P: 1-100-1-200, IF/ICC: 1-100-1-500, IP: 1-10-1-100
• Reactivity: Human, Monkey, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Creatine Kinase M. The exact sequence is proprietary.
• Target: CKM
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CKMM; Creatine kinase M-type; Creatine kinase M chain; M-CK

Immunofluorescent analysis of Creatine Kinase M staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Western blot analysis of mouse spleen(1), rat liver (2), rat heart (3), mouse lung (4) using Creatine Kinase M Rabbit Polyclonal Antibody orb213745 with dilution at 1:1000.

Immunohistochemical analysis of paraffin-embedded mouse brain using Creatine Kinase M Rabbit Polyclonal Antibody orb213745 with dilution at 1:100.

Immunohistochemical analysis of paraffin-embedded rat lung using Creatine Kinase M Rabbit Polyclonal Antibody orb213745 with dilution at 1:100.

Immunohistochemical analysis of paraffin-embedded rat lung using Creatine Kinase M Rabbit Polyclonal Antibody orb213745 with dilution at 1:100.

Immunohistochemical analysis of paraffin-embedded rat stomach using Creatine Kinase M Rabbit Polyclonal Antibody orb213745 with dilution at 1:100.

Quick Database Links

Gene Symbol

CKM

UniProt

P07310, P06732, P00564

NCBI Gene ID

1158, 24265, 12715