CKM Antibody (C-term)

SKU: orb1929585

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Signal Transduction

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Dilution Range: WB - 1:1000, IHC-P - 1:50-100, FC - 1:10-50
• Reactivity: Human
• Predicted Reactivity: Porcine, Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: This CKM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 283-313 amino acids from the C-terminal region of human CKM. Antigen Region: 283-313 aa.
• Target: CKM
• Molecular Weight: 43101 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Creatine kinase M-type, Creatine kinase M chain, M-CK, Creatine kinase M-type, N-terminally processed, CKM, CKMM

Flow cytometric analysis of HepG2 cells using Creatine Kinase MB (CKM) Antibody (C-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human skeletal muscle reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

The anti-CKM Pab is used in Western blot to detect CKM in C6 cell lysate.

Quick Database Links

Gene Symbol

CKM

UniProt

P06732

RefSeq (Protein):NP_001815.2