COX4-1 Mouse Monoclonal Antibody

SKU: orb1474397

Description

The COX4-1 Antibody is suitable for FC, IF, IHC, IP, WB. It is a Monoclonal, Unconjugated antibody which raised against A synthetic peptide corresponding to carboxyl terminal residues of human COX IV .Purification: The antibody was purified by immunogen affinity chromatography.

Images & Validation

• Tested Applications: FC, IF, IHC, IP, WB
• Dilution Range: WB (1/500 - 1/1000), IH (1/50 - 1/100), IF/IC (1/50 - 1/100), IP (1/10 - 1/50), FC (1/50 - 1/100)
• Reactivity: Goat, Hamster, Human, Monkey, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Immunogen: A synthetic peptide corresponding to carboxyl terminal residues of human COX IV
• Target: COX4I1
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

COX4; Cytochrome c oxidase subunit 4 isoform 1 mitochondrial; Cytochrome c oxidase polypeptide IV; Cytochrome c oxidase subunit IV isoform 1; COX IV-1

Western blot analysis of COX4-1 expression in CHOk1 (A), COS7 (B), NIH3T3 (C), Hela (D), C6 (E), mouse skeletal muscel (F) whole cell lysates. (Predicted band size: 20 kD; Observed band size: 17 kD)

Immunohistochemical analysis of COX4-1 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.50). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of COX4-1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.

Flow cytometric analysis of COX4-1 in K562 (red). Blue line histogram represents the isotype control.

Quick Database Links

Gene Symbol

COX4I1

UniProt

P19783, P10888, P13073

NCBI Gene ID

1327, 12857, 29445