Anti-Cleaved PARP1 Antibody

• Catalog Number: orb323122
• Category: Antibodies
• Description: Mouse monoclonal antibody to Cleaved PARP1
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: IH, WB
• Reactivity: Human
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence of human Cleaved PARP1. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:2000-1:5000, IHC-P: 1:200-1:500
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: PARP1
• Entrez: 142
• UniProt ID: P09874
• Source: Mouse
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti ADPRT antibody, anti PPOL antibody, anti Poly
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Wang, Weiwei et al. Notoginsenoside R1 alleviates high glucose-evoked damage in RSC96 cells through down-regulation of miR-503 Artif Cells Nanomed Biotechnol, 47, 3947-3954 (2019)

PubMed: 31581849

Western blot analysis of Cleaved PARP1 expression in Jurkat (A) whole cell lysates. (Predicted band size: 113 kD; Observed band size: 116, 89 kD)

Immunohistochemical analysis of Cleaved PARP1 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.