Cleaved PARP1 Mouse Monoclonal Antibody

SKU: orb499776

Description

Cleaved PARP1 Mouse Monoclonal Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P
• Dilution Range: IHC-P=1:200-800, IHC-F=1:500-1000, IF=1:500-1000
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: 4B1
• Immunogen: KLH conjugated synthetic peptide derived from human PARP
• Target: PARP1
• Molecular Weight: 89/116 kDa
• Purification: Affinity purified by Protein G
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ADPRT; ADPRT 1; ADPRT1; ARTD1; PARP; PARP-1; PARS; PPOL; Poly-PARP; pADPRT-1; 5830444G22Rik; Adprp; msPARP; sPARP-1; PARP1_HUMAN; PARP1; ADP-ribosyltransferase diphtheria toxin-like 1 (ARTD1); DNA ADP-ribosyltransferase PARP1; NAD(+) ADP-ribosyltransferase 1 (ADPRT 1); Poly[ADP-ribose] synthase 1; Protein poly-ADP-ribosyltransferase PARP1; 2.4.2.30; PARP1_MOUSE; Poly[ADP-ribose] synthase 1 (msPARP); PARP1_RAT;

Paraformaldehyde-fixed, paraffin embedded (Human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Cleaved PARP) Monoclonal Antibody, Unconjugated (orb499776 4B1) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Quick Database Links

Gene Symbol

PARP1

UniProt

P09874