Anti-CK1 alpha (Phospho-Y294) Antibody

• Catalog Number: orb393259
• Category: Antibodies
• Description: Rabbit polyclonal antibody to CSNK1A1 (Phospho-Y294)
• Target: CSNK1A1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rabbit, Rat, Sheep
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y294 of human CK1 alpha protein. The exact sequence is proprietary.
• UniProt ID: P48729, Q8BK63, Q8N752, P97633
• Tested applications: IH, WB
• Dilution range: WB: 1:500:1000, IHC-P: 1:100:200
• Antibody Type: Primary Antibody
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CSNK1A1; Casein kinase I isoform alpha; CKI-alpha;
• Note: For research use only
• Entrez: 113927, 122011, 1452

Western blot analysis of CK1 alpha (Phospho-Y294) expression in Jurkat heat shock-treated (A) whole cell lysates. (Predicted band size: 38; 39 kD; Observed band size: 38 kD)

Immunohistochemical analysis of CK1 alpha (Phospho-Y294) staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.