CK1 alpha (Phospho-Y294) Antibody

SKU: orb393259

Description

The CK1 alpha (Phospho-Y294) Antibody is suitable for IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y294 of human CK1 alpha protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Research Area

Epigenetics, Neuroscience, Signaling Pathways, Wnt

Images & Validation

• Tested Applications: IHC, WB
• Dilution range: WB: 1:500:1000, IHC-P: 1:100:200
• Reactivity: Bovine, Gallus, Human, Mouse, Rabbit, Rat, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y294 of human CK1 alpha protein. The exact sequence is proprietary.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Disclaimer: For research use only

Alternative Names

CSNK1A1; Casein kinase I isoform alpha; CKI-alpha; CK1; CSNK1A1L; Casein kinase I isoform alpha-like; CKI-alpha-like; CK1

Western blot analysis of CK1 alpha (Phospho-Y294) expression in Jurkat heat shock-treated (A) whole cell lysates. (Predicted band size: 38; 39 kD; Observed band size: 38 kD)

Immunohistochemical analysis of CK1 alpha (Phospho-Y294) staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Quick Database Links

UniProt

P97633, Q8N752, P48729, Q8BK63

NCBI Gene ID

122011, 113927, 1452