Anti-CDC6 (Phospho-S54) Antibody

• Catalog Number: orb304718
• Category: Antibodies
• Description: Rabbit polyclonal antibody to CDC6 (phospho-S54)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Primate, Rat
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S54 of human CDC6 protein. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:500-1000, IHC-P: 1:100-200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: CDC6
• Entrez: 990, 23834
• UniProt ID: O89033, Q99741
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti CDC18L antibody, anti Cell division control p
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of CDC6 (Phospho-S54) expression in HeLa serum starvation-treated (A), Raw264.7 serum starvation-treated (B), H9C2 serum starvation-treated (C) whole cell lysates. (Predicted band size: 62 kD; Observed band size: 62 kD)

Immunohistochemical analysis of CDC6 (Phospho-S54) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.