Phospho-Cdc6 (Ser54) Rabbit Polyclonal Antibody

• Catalog Number: orb4867
• Category: Antibodies
• Description: Phospho-Cdc6 (Ser54) Rabbit Polyclonal Antibody
• Target: CDC6
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Canine, Equine, Mouse, Porcine, Rabbit, Rat, Sheep
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human Cdc6 around the phosphorylation site of Ser54 PL(p-S)PR
• UniProt ID: Q99741
• RRID: AB_10937261
• MW: 63 kDa
• Tested applications: ICC
• Dilution range: ICC/IF=1:50-200
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Cell Division Cycle protein 6; Cdc 18L; Cdc 6; CDC
• Research Area: Cell Biology, Epigenetics and Nuclear Signaling
• Note: For research use only

HUVEC cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (phospho-Cdc6 (Ser54)) polyclonal Antibody, Unconjugated (orb4867) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.