Anti-CD79B antibody(52A11); IgG1 Chimeric mAb

• Catalog Number: orb1826934
• Category: Antibodies
• Description: Anti-CD79B antibody(52A11); IgG1 Chimeric mAb
• Target: CD79B
• Clonality: Monoclonal
• Species/Host: Rabbit
• Isotype: Rabbit/Human Fc chimeric IgG1
• Conjugation: Unconjugated
• Reactivity: Human
• UniProt ID: P40259
• Tested applications: FC, WB
• Dilution range: Flow Cyt 1:100; WB 1:1000
• Application notes: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Antibody Type: Recombinant Antibody
• Clone Number: 52A11
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: AGM6;B29;IGB
• Note: For research use only

Flow cytometry analysis with Anti-CD79B (52A11) mAb on Expi293 cells transfected with human CD79B (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).

Flow cytometry analysis of antigen binding of anti-human CD79B mAb (orb1826934). (A) orb1826934 does not bind to 293T cells that do not express CD79B. (B) A clear peak shift of orb1826934 was seen compared to the control when incubated with CD79B-expressing Raji cells, indicating strong binding of orb1826934 to CD79B. Antibodies were incubated at 5 μg/mL.

Anti-CD79B antibody (SKU# orb1826934) at 1/1000 dilution.Lane: RAJI (human Burkitt's lymphoma B lymphocyte), whole cell lysate.Secondary: Goat Anti-Rabbit IgG H&L (HRP) at 1/5000 dilution.Predicted band size: 26 kDa.Observed band size: 24 kDa