Anti-CD47 antibody(DM212); Rabbit mAb

• Catalog Number: orb1291136
• Category: Antibodies
• Description: Anti-CD47 antibody(DM212), Rabbit mAb
• Target: CD47
• Clonality: Monoclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• UniProt ID: Q08722
• Tested applications: ELISA, FC
• Dilution range: ELISA 1/5000-10000|Flow Cyt 1/100
• Application notes: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Antibody Type: Recombinant Antibody
• Clone Number: DM212
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CD47; MER6; IAP; OA3
• Note: For research use only

Competition assay demonstrating DM212 blockade of SIRPα binding to Jurkat cell line.

Flow cytometry analysis of antigen binding of rabbit anti-human CD47 mAb (orb1291136). (A) orb1291136 does not bind to CHO-S cells that do not express CD47. (B) A clear peak shift of orb1291136 was seen compared to the control when incubated with CD47-expressing SNU-5 cells, indicating strong binding of orb1291136 to CD47. Antibodies were incubated at 5 μg/ml.

Flow cytometry analysis of antigen binding of rabbit anti-human CD47 mAb (orb1291136). (A) orb1291136 does not bind to CHO-S cells that do not express CD47. (B) A clear peak shift of orb1291136 was seen compared to the control when incubated with CD47-expressing 8226 cells, indicating strong binding of orb1291136 to CD47. Antibodies were incubated at 5 μg/ml.