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CD38 Antibody
Description
Images & Validation
−| Tested Applications | ELISA, FC, IHC, WB |
|---|---|
| Dilution Range | ELISA 1/5000-10000| Flow Cyt 1/100 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Recombinant Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | Rabbit IgG |
| Clone No. | DM28 |
| Target | CD38 |
| Purification | Purified from cell culture supernatant by affinity chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Expi 293 cell line transfected with irrelevant protein (left) and human CD38 (right) were surface stained with Rabbit anti-CD38 monoclonal antibody 1 μg/ml (clone: DM28) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.

Flow cytometry analysis of antigen binding of rabbit anti-human CD38 mAb (orb689348). (A) orb689348 does not bind to 293T cells that do not express CD38. (B) A clear peak shift of orb689348 was seen compared to the control when incubated with CD38-expressing H929 cells, indicating strong binding of orb689348 to CD38. Antibodies were incubated at 5 μg/mL.

A. orb689348 at 5 μg/ml staining CD38 in RPMI-8226 MM xenografts in NSG mice by IHC (SKU# orb689348); B: orb689348 at 5 μg/ml staining CD38 in human tonsil tissue by IHC (SKU# orb689348);

Western blot analysis of CD38 protein using Anti-CD38 antibody (Cat. orb689348) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H L) at 1:5000 dilution.1. MM.1S cell lysate (native CD38 protein).2. H929 cell lysate (native CD38 protein)

A. orb689348 at 10 μg/ml staining CD38 in M-NSG Daudi DiSliceX SlideSet section by IHC (SKU# orb689348); B. orb689348 at 10 μg/ml staining CD38 in human tonsil tissue by IHC (SKU# orb689348).
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CD38 Antibody (orb689348)
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