Anti-CD38 antibody(DM28); Rabbit mAb

• Catalog Number: orb689348
• Category: Antibodies
• Description: Anti-CD38 antibody(DM28), Rabbit mAb
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: DM28
• Tested applications: ELISA, FC, IHC, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Recombinant Antibody
• Dilution range: ELISA 1/5000-10000| Flow Cyt 1/100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: CD38
• UniProt ID: P28907
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: T10, cADPr hydrolase 1
• Note: For research use only
• Application notes: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Expiration Date: 12 months from date of receipt.

Expi 293 cell line transfected with irrelevant protein (left) and human CD38 (right) were surface stained with Rabbit anti-CD38 monoclonal antibody 1 μg/ml (clone: DM28) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.

Flow cytometry analysis of antigen binding of rabbit anti-human CD38 mAb (orb689348). (A) orb689348 does not bind to 293T cells that do not express CD38. (B) A clear peak shift of orb689348 was seen compared to the control when incubated with CD38-expressing H929 cells, indicating strong binding of orb689348 to CD38. Antibodies were incubated at 5 μg/mL.

A. orb689348 at 5 μg/ml staining CD38 in RPMI-8226 MM xenografts in NSG mice by IHC (SKU# orb689348); B: orb689348 at 5 μg/ml staining CD38 in human tonsil tissue by IHC (SKU# orb689348);

Western blot analysis of CD38 protein using Anti-CD38 antibody (Cat. orb689348) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H L) at 1:5000 dilution.1. MM.1S cell lysate (native CD38 protein).2. H929 cell lysate (native CD38 protein)

A. orb689348 at 10 μg/ml staining CD38 in M-NSG Daudi DiSliceX SlideSet section by IHC (SKU# orb689348); B. orb689348 at 10 μg/ml staining CD38 in human tonsil tissue by IHC (SKU# orb689348).