CD38 Antibody (C-term)

SKU: orb1931372

Description

CD38 Antibody (C-term)

Research Area

Cancer Biology, Immunology & Inflammation, Metabolism Research, Signal Transduction

Images & Validation

• Tested Applications: FC, IF, IHC-P, WB
• Dilution range: WB - 1000, IHC-P - 1:100-500, IF - 1:10-50, FC - 1:10-50
• Reactivity: Human
• Predicted Reactivity: Monkey

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 34328 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Disclaimer: For research use only

Flow cytometric analysis of HepG2 cells using CD38 Antibody (C-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of CD38 Antibody (C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Immunofluorescence analysis of CD38 Antibody (C-term) with paraffin-embedded human hepatocarcinoma tissue.0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.

Formalin-fixed and paraffin-embedded human hepatocarcinoma with CD38 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot analysis of lysate from RPMI 8226 cell line, using CD38 Antibody (C-term). Diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35 ug.

Western blot analysis of lysate from RPMI8226 cell line, using CD38 Antibody (C-term). Diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20 ug.

Western blot analysis of lysate from 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CD38 gene using CD38 Antibody (C-term). Diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20 ug.

Quick Database Links

UniProt

P28907