Anti-CD30 antibody(DM105); Rabbit mAb

• Catalog Number: orb1174063
• Category: Antibodies
• Description: Anti-CD30 antibody(DM105), Rabbit mAb
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: DM105
• Tested applications: ELISA, FC, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Recombinant Antibody
• Dilution range: ELISA 1/5000-10000|Flow Cyt 1/100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: CD30
• UniProt ID: P28908
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: TNFRSF8;CD30;D1S166E;Ki-1
• Note: For research use only
• Application notes: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Expiration Date: 12 months from date of receipt.

ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human CD30 protein, His tagged protein orb689465 can bind Rabbit anti-CD30 monoclonal antibody (clone: DM105) in a linear range of 0.12-80 ng/ml.

Flow cytometry analysis with Anti-CD30 on Expi293 cells transfected with human CD30 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).

Flow cytometry analysis of antigen binding of rabbit anti-human CD30 mAb (orb1174063). (A) orb1174063 does not bind to 293T cells that do not express CD30. (B) A clear peak shift of orb1174063 was seen compared to the control when incubated with CD30-expressing 8226 cells, indicating strong binding of orb1174063 to CD30. Antibodies were incubated at 10 μg/mL.

Anti-CD30 antibody (SKU# orb1174063) at 1/1000 dilution.Lane: RPMI8226, whole cell lysate.Secondary: Goat Anti-Rabbit IgG H&L (HRP) at 1/5000 dilution.Predicted band size: 64 kDa.Observed band size: 90, 110 kDa