Anti-CD25 [PC-61.5.3]

SKU: orb758826

Description

Rat monoclonal antibody to CD25

Images & Validation

• Tested Applications: ELISA, FA, FC, IF, WB
• Reactivity: Mouse

Key Properties

• Host: Rat
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone No.: PC-61.5.3
• Immunogen: This antibody was raised by immunising rats with the B6.1 mouse cytotoxic T cell line, followed by fusing spleen cells with P3X63Ag8.653 myeloma cells.
• Target: CD25
• Purity: Purified

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

IL2-RA; IL-2 receptor subunit alpha;L-2R alpha chain; IL-2R subunit alpha; Interleukin-2 receptor subunit alpha; p55; p55 chain; PC61; PC6153

Immunofluorescence staining of fixed mouse splenocytes with anti-CD25 antibody PC-61.5.3. Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of PC-61.5.3 (orb758825) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb758825, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256449) followed by staining with Alexa Fluor® 488 secondary antibody.

Quick Database Links

Gene Symbol

CD25

UniProt

P01590