Anti-CD25 [PC-61.5.3]

• Catalog Number: orb758825
• Category: Antibodies
• Description: Rabbit monoclonal antibody to CD25
• Target: CD25
• Clonality: Monoclonal
• Species/Host: Rat
• Isotype: Rat IgG
• Conjugation: Unconjugated
• Reactivity: Mouse
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Purity: Purified
• Immunogen: This antibody was raised by immunising rats with the B6.1 mouse cytotoxic T cell line, followed by fusing spleen cells with P3X63Ag8.653 myeloma cells.
• UniProt ID: P01590
• Tested applications: ELISA, FA, FC, IF, WB
• Clone Number: PC-61.5.3
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: IL2-RA; IL-2 receptor subunit alpha;L-2R alpha cha
• Note: For research use only

Immunofluorescence staining of fixed mouse splenocytes with anti-CD25 antibody PC-61.5.3. Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of PC-61.5.3 (orb758825) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb758825, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256449) followed by staining with Alexa Fluor® 488 secondary antibody.