Anti-CD24 antibody(DMC213); IgG1 Chimeric mAb

• Catalog Number: orb1291204
• Category: Antibodies
• Description: Anti-CD24 antibody(DMC213), IgG1 Chimeric mAb
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: DMC213
• Tested applications: ELISA, FC, IHC, WB
• Reactivity: Human
• Isotype: Rabbit/Human Fc chimeric IgG1
• Antibody Type: Recombinant Antibody
• Dilution range: ELISA 1/5000-10000|Flow Cyt 1/100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: CD24
• UniProt ID: P25063
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CD24A
• Note: For research use only
• Application notes: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Expiration Date: 12 months from date of receipt.

Flow cytometry analysis with Anti-CD24 on Expi293 cells transfected with human CD24 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).

Anti-CD24 antibody (SKU# orb1291204) at 1/1000 dilution.Lane: Huh7, whole cell lysate.Secondary: Goat Anti-Rabbit IgG H&L (HRP) at 1/5000 dilution.Predicted band size: 61 kDa.Observed band size: 25, 135 kDa

A. orb1291204 at 10 μg/ml staining CD24 in K562-CD24 cells by IHC (SKU# orb1291204); B. orb1291204 at 10 μg/ml staining CD24 in human tonsil tissue by IHC (SKU# orb1291204)

Flow cytometry analysis of antigen binding of anti-human CD24 mAb (orb1291204). (A) orb1291204 does not bind to Jurkat cells that do not express CD24. (B) A clear peak shift of orb1291204 was seen compared to the control when incubated with CD24-expressing MCF-7 cells, indicating strong binding of orb1291204 to CD24. Antibodies were incubated at 10 μg/mL.