CD24 Antibody (N-Term)

• Catalog Number: orb2649267
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB23639
• Tested applications: FC, IHC-P, WB
• Predicted Reactivity: Mouse
• Reactivity: Human
• Isotype: Rabbit IgG
• Dilution range: WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 8083 Da
• Target: This CD24 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human CD24.
• UniProt ID: P25063
• NCBI: NP_037362.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Signal transducer CD24, Small cell lung carcinoma
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of CD24 Antibody (N-Term) in MCF-7 cell line lysates (35 ug/lane). CD24 (arrow) was detected using the purified Pab.

CD24 Antibody (N-Term) western blot analysis in Raji, Ramos cell line and rat cerebellum tissue lysates (35 ug/lane). This demonstrates the CD24 antibody detected the CD24 protein (arrow).

CD24 Antibody (N-Term) flow cytometry analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human breast carcinoma reacted with CD24 Antibody (N-Term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.