CD24 Antibody (N-Term)

• Catalog Number: orb2649267
• Category: Antibodies
• Description: CD24 Antibody (N-Term)
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Mouse
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: P25063
• MW: 8083 Da
• Tested applications: FC, IHC-P, WB
• Dilution range: WB - 1:500, IHC-P - 1:100-500, FC - 1:10-50
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CD24A
• Research Area: Cancer Biology, Immunology & Inflammation, Metabol
• Note: For research use only

Western blot analysis of CD24 Antibody (N-Term) in MCF-7 cell line lysates (35 ug/lane). CD24 (arrow) was detected using the purified Pab.

CD24 Antibody (N-Term) western blot analysis in Raji, Ramos cell line and rat cerebellum tissue lysates (35 ug/lane). This demonstrates the CD24 antibody detected the CD24 protein (arrow).

CD24 Antibody (N-Term) flow cytometry analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human breast carcinoma reacted with CD24 Antibody (N-Term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.