CD163 Antibody

• Catalog Number: orb412707
• Category: Antibodies
• Description: Rabbit polyclonal antibody to CD163
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: Recombinant fusion protein of human CD163
• UniProt ID: Q2VLH6, Q86VB7
• Tested applications: IF, IHC, WB
• Dilution range: WB: 1:500-2000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: M130; Scavenger receptor cysteine-rich type 1 prot
• Research Area: Epigenetics, Immunology
• Note: For research use only
• Entrez: 9332, 93671
• Expiration Date: 12 months from date of receipt.

Western blot analysis of CD163 expression in SW480 (A), mouse lung (B), rat liver (C) whole cell lysates. (Predicted band size: 121; 124; 125 kD; Observed band size: 150 kD)

Immunohistochemical analysis of CD163 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of CD163 staining in THP1 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).