Anti-CD112 antibody(DMC224); IgG1 Chimeric mAb

• Catalog Number: orb1291197
• Category: Antibodies
• Description: Anti-CD112 antibody(DMC224), IgG1 Chimeric mAb
• Target: CD112
• Clonality: Monoclonal
• Species/Host: Rabbit
• Isotype: Rabbit/Human Fc chimeric IgG1
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Purification: Purified from cell culture supernatant by affinity chromatography
• UniProt ID: Q92692
• Tested applications: ELISA, FC
• Dilution range: ELISA 1/5000-10000|Flow Cyt 1/100
• Application notes: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Antibody Type: Recombinant Antibody
• Clone Number: DMC224
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: NECTIN2; HVEB; PRR2; PVRL2; PVRR2
• Note: For research use only

CD112 protein is highly expressed on the surface of Expi293 cell membrane. Flow cytometry analysis with Anti-CD112 on Expi293 cells transfected with human CD112 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram), and Isotype antibody on Expi293 transfected with irrelevant protein (Orange histogram).

Flow cytometry analysis of antigen binding of anti-human CD112 mAb (orb1291197). (A) orb1291197 does not bind to MM.1S cells that do not express CD112. (B) A clear peak shift of orb1291197 was seen compared to the control when incubated with CD112-expressing MCF-7 cells, indicating strong binding of orb1291197 to CD112. Antibodies were incubated at 5 μg/mL.

Flow cytometry analysis of antigen binding of anti-human CD112 mAb (orb1291197). (A) orb1291197 does not bind to MM.1S cells that do not express CD112. (B) A clear peak shift of orb1291197 was seen compared to the control when incubated with CD112-expressing Huh7 cells, indicating strong binding of orb1291197 to CD112. Antibodies were incubated at 5 μg/mL.